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SRX8646676: ONT SQK-RAD004 library of YALI2_DNA_HMW gDNA for ultra-long reads: R9.5 flowcell
1 OXFORD_NANOPORE (MinION) run: 48,717 spots, 1.7G bases, 1.3Gb downloads

Design: Genomic DNA was isolated using the the NucleoBond HMW DNA kit (Macherey-Nagel) with an adapted protocol as described in Luttermann et al. (to be published). The sequencing library was prepared using a Rapid Sequencing Kit (SQK-RAD004, Oxford Nanopore Technologies) with changes as described in Luttermann et al. (to be published). Sequencing was performed using a R9.5 flowcell on a MinION sequencing platform according to the manufacturers instructions. Basecalling and demultiplexing were performed using albacore v2.3.1 and porechop v0.2.3.
Submitted by: Uni Bielefeld
Study: Yarrowia lipolytica DSM 3286 Genome sequencing, assembly, and improved annotation
show Abstracthide Abstract
Complete genome sequence of Yarrowia lipolytica DSM 3286 with partial resolution of rDNA clusters and telomeric repeats as well as annotation of 5'-UTRs
Sample: Yarrowia lipolytica DSM 3286 cells for the isolation of high molecular weight genomic DNA
SAMN15366429 • SRS6930219 • All experiments • All runs
Library:
Name: YALI2_DNA_HMW_ONT_RAD_ULR
Instrument: MinION
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: SINGLE
Runs: 1 run, 48,717 spots, 1.7G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR1212537148,7171.7G1.3Gb2020-07-01

ID:
11245538

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